This course extends the knowledge of the analytical chemistry to include the fundamental principles and application of modern instrumental methods including spectroscopic, spectrometric, and separation methods. The topics covered have applications to many subject areas in Natural Sciences, Life Science and Engineering.
This course is presented in a lecture/discussion format. The content of the course includes:
Part I:
I. Potentiometry
A) Analysis Review
B) Indicator electrode
a) Glass pH electrode
b) Ion-selective electrode (ISE)
c) Ion-selective field effect transistor
II. Electrolysis
A. Electrogravimetry
a) Analysis and Electrode
b) Separation
c) Non-potentiostatic Method
d) Potentiostatic Method
B. Coulometry
III. Amperometry
A. Analysis
B. Examples
IV. Voltammetry
A. Applied Potential, Eappl
a) Linear Sweep
b) Staircase
c) Square Wave
B. Current, i
C. Basis of Analysis
a) Faradaic and Charging Currents
b) Supporting Electrolyte
c) Vitamin C Analysis
D. Cyclic Voltammetry
E. Sampled Current Polarography
F. Square Wave Voltammetry
G. Stripping Method

Part II:
I. Introduction to Instrumental Analysis
II. Fundamentals of Spectroscopy/Spectrometry
A. Introduction to Spectroscopy
B. Characteristics of Electromagnetic radiation
C. Wave Property of Light and Optical Component
a) Interference
b) Transmission
c) Reflection
d) Refraction
e) Scattering
f) Diffraction
D. Particle Property of Light and Its Use
a) Photoelectric effect
b) Absorption
c) Emission
III. Atomic Spectroscopy
A. Principle
a) Emission
b) Atomic Structure
B. Measurement
a) Source of Errors
b) Spectral Line Shape
c) Temperature Effect
d) Interference
C. Instrumentation
a) Main Components
b) Sources
IV. UV and Visible Absorption Spectrometry
A. Range and Type of UV/Vis Radiation
B. Principle
a) Molar Absorptivity
b) Spectroscopic Process
c) Effect of Multichromophores
d) d, f-electron Transition
C. Measurement
a) Standard Addition Method
b) Solvent Selection
c) Solvent Effect
d) Beer’s Law and Its Limitations
e) Spectrophotometric Uncertainty
D. Instrumentation
a) Single-Beam and Double-Beam Instruments
b) Sample Cell and Construction Materials
c) Source
d) Slit
e) Detector
E. Spectroscopic Information
a) Imaging
b) Qualitative
c) Quantitative
F. Spectral Analysis
a) Woodward-Fieser Rules
V. Luminescence Spectroscopy
A. Luminescence
B. Principle
a) Spectroscopic Process
b) Quantum Yield
c) Favorable Condition for Luminescence
d) Emission Spectrum
e) 90˚ Detection
f) Luminescence Intensity
g) Type of Luminescence
C. Instrumentation
D. Spectroscopic Information
a) FL System
b) Fluorescent Agent
c) Use of Excited-State Molecules
d) Fluorescence Resonance Energy Transfer
VI. Infrared Spectroscopy
A. Range and Type of IR Radiation
B. Principle
a) Molecular Excitation
b) Type of Molecular Vibration
c) Normal Mode of Vibration
d) Spectroscopic Process
e) IR-Active Vibration
C. Instrumentation
a) Nondispersive Instrument
b) Dispersive Instrument
c) Sample Cell and Construction Materials
d) Source
e) Wavelength Selector
f) Detector
D. Spectroscopic Information
a) Functional Group
b) Quantitative
VII. Raman Spectroscopy
A. Principle
a) Polarizability
b) Raman Scattering
c) Energy Shift
d) Raman-Active Vibration
e) Raman Scattering Intensity
f) Advantage Over IR
B. Instrumentation
a) Laser
C. Spectroscopic Information
D. Raman vs. IR
VIII. Nuclear Magnetic Resonance Spectroscopy
A. Principle
a) Range of Radiation
b) Nuclear Spin
B. Instrumentation and Measurement
a) Phase Coherence
b) Magnetic Resonance
c) Secondary Field
IX. Mass Spectrometry
A. Ionization Method
a) Gas-Phase Ionization
b) Liquid-Phase Ionization
c) Solid-Phase Ionization
B. Mass Analyzer
a) Double Focusing
b) Time-of-Flight
c) Quadrupole
d) Ion Trap
C. Detector
a) Faraday Cup
b) Channel Electron Multiplier
c) Multichannel Plate
D. Spectral Analysis
X. Analytical Separation
A. General Description
a) Solvent Extraction
b) Mobile and Stationary Phases
c) Classification
d) Elution
B. Principle
a) Migration Rate
b) Retention Time
c) Flow Rate – Flow Velocity Relationship
d) Migration Rate - Distribution Constant
e) Retention Factor
f) Selectivity Factor
g) Band Broadening
h) Column Efficiency
i) Column Resolution
j) Column Resolution